Lyophilized E. coli-based Cell-Free Systems for Protein Synthesis ID: 2013-090

This invention significantly enhances cell-free protein synthesis (CFPS) through the development of lyophilized Escherichia coli extracts for robust, efficient storage and transport.

Technology Overview

The technology involves the lyophilization, or freeze-drying, of E. coli cell extracts to create a powdered form that retains protein synthesis activity for extended periods at room temperature. This method overcomes the limitations of traditional liquid cell extracts, which require ultra-low temperature storage, by offering a high-density, long-term storage solution that is more practical and cost-effective.

Key Advantages

• Enables long-term storage of cell-free systems at room temperature
• Eliminates the need for ultra-low temperature storage and transport
• Increases the viability and efficiency of protein synthesis over extended periods
• Facilitates high-density storage, reducing space and cost requirements
• Supports the development of portable protein production technologies

Problems Addressed

• Instability and short shelf life of aqueous cell-free protein synthesis extracts
• High costs and logistical challenges associated with ultra-low temperature storage
• Limited applicability of CFPS in field settings due to storage constraints

Market Applications

• Pharmacy-on-a-chip devices for on-demand drug synthesis
• Portable diagnostic kits utilizing protein markers
• Educational kits for synthetic biology and bioengineering
• Research tools for protein engineering and synthetic biology
• Biomanufacturing, especially in remote or resource-limited settings

Additional Information

Technology ID: 2013-090
Sell Sheet: Download the Sell Sheet here
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Date Published: 13 May, 2025